Backbone dynamics of a winged helix protein and its DNA complex at different temperatures: changes of internal motions in genesis upon binding to DNA
- 影响因子：
0.0
- 发表刊物：
J Mol Biol.
- 摘要：
The dynamic properties of a winged helix protein, Genesis, and its DNA complex at different temperatures were studied. Due to the complexity of motions, the commonly used model-free formalism could not be used to reflect the dynamic properties. The reduced spectral density function mapping approach was proven to be a useful tool to describe the overall and internal motion of molecules on the picosecond to nanosecond time-scale, and conformational exchanges on the microsecond to millisecond time-scale. The local motions in DNA-free Genesis showed strong temperature dependence and the backbone dynamics of each secondary structural element responds to the temperature change differently, while the Genesis-DNA complex showed more stability with changing the temperatures. Furthermore, each DNA contact sequence of Genesis showed distinct dynamic perturbation after Genesis binds to DNA.
- 论文类型：
期刊论文
- 卷号：
292
- 期号：
3
- 页面范围：
641-651
- 是否译文：
否
- 发表时间：
1999-09-24
- 收录刊物：
SCI
- 发布期刊链接：
- 第一作者：
Jin Changwen
- 通讯作者：
Liao Xiubei
