Dynamic DNA contacts observed in the NMR structure of winged helix protein-DNA complex
- 影响因子：
0.0
- 发表刊物：
J. Mol. Biol. (Communication)
- 摘要：
Genesis is an HNF-3/fkh homologous protein. By using multi-dimensional NMR techniques, we have obtained the solution structure and backbone dynamics of Genesis complexed with a 17 base-pair DNA. Our results indicate that both the local folding and dynamic properties of Genesis are perturbed when it binds to the DNA site. Our data show that a conserved flexible amino acid sequence (wing 1) makes dynamic contacts to DNA in the complex and a short helix is induced by Genesis-DNA interactions. Our data indicate that, unlike the HNF-3gamma/DNA complex, a magnesium ion is not required in forming the stable Genesis-DNA complex.
- 论文类型：
期刊论文
- 卷号：
289
- 期号：
4
- 页面范围：
683-690
- 是否译文：
否
- 发表时间：
1999-06-18
- 收录刊物：
SCI
- 发布期刊链接：
- 第一作者：
Jin Changwen
- 通讯作者：
Liao Xiubei
- 全部作者：
Chen Xiaojun,Marsden Lan
