- 影响因子：
0.0
- 发表刊物：
J Biol Chem.
- 关键字：
Cdc18; DNA; DNA binding protein; DNA origin; DNA replication; DNA replication initiation
- 摘要：
A central step in the initiation of chromosomal DNA replication in eukaryotes is the assembly of pre-replicative complex (pre-RC) at late M and early G1 phase of the cell cycles. Since 1973, four proteins or protein complexes, including cell division control protein 6 (Cdc6)/Cdc18, minichromosome maintenance protein complex, origin recognition complex (ORC), and Cdt1, are known components of the pre-RC. Previously, we reported that a non-ORC protein binds to the essential element Δ9 of the Schizosaccharomyces pombe DNA-replication origin ARS3001. In this study, we identified that the non-ORC protein is Sap1. Like ORC, Sap1 binds to DNA origins during cell growth cycles. But unlike ORC, which binds to asymmetric AT-rich sequences through its nine AT-hook motifs, Sap1 preferentially binds to a DNA sequence of 5'-(A/T) n (C/G)(A/T)9-10(G/C)(A/T) n -3' (n ≥ 1). We also found that Sap1 and ORC physically interact. We further demonstrated that Sap1 is required for the assembly of the pre-RC because of its essential role in recruiting Cdc18 to DNA origins. Thus, we conclude that Sap1 is a replication-initiation factor that directly participates in the assembly of the pre-RC. DNA-replication origins in fission yeast are defined by possessing two essential elements with one bound by ORC and the other by Sap1.
- 论文类型：
期刊论文
- 论文编号：
53
- 卷号：
292
- 期号：
15
- 页面范围：
6056-6075
- 是否译文：
否
- 发表时间：
2017-04-14
- 收录刊物：
SCI
- 发布期刊链接：
- 第一作者：
Guan Ling
- 通讯作者：
Jin Changwen,Kong Daochun
- 全部作者：
He Peng,Yang Fang,Zhang Yuan,Hu Yunfei,Ding Jienv,Hua Yu,Zhang Yi,Ye Qiong,Hu Jiazhi,Wang Tao
