The solution structure of Escherichia coli Wzb reveals a novel substrate recognition mechanism of prokaryotic low molecular weight protein tyrosin phophatases
- 影响因子：
0.0
- 发表刊物：
J. Biol. Chem
- 摘要：
Low molecular weight protein-tyrosine phosphatases (LMW-PTPs) are small enzymes that ubiquitously exist in various organisms and play important roles in many biological processes. In Escherichia coli, the LMW-PTP Wzb dephosphorylates the autokinase Wzc, and the Wzc/Wzb pair regulates colanic acid production. However, the substrate recognition mechanism of Wzb is still poorly understood thus far. To elucidate the molecular basis of the catalytic mechanism, we have determined the solution structure of Wzb at high resolution by NMR spectroscopy. The Wzb structure highly resembles that of the typical LMW-PTP fold, suggesting that Wzb may adopt a similar catalytic mechanism with other LMW-PTPs. Nevertheless, in comparison with eukaryotic LMW-PTPs, the absence of an aromatic amino acid at the bottom of the active site significantly alters the molecular surface and implicates Wzb may adopt a novel substrate recognition mechanism. Furthermore, a structure-based multiple sequence alignment suggests that a class of the prokaryotic LMW-PTPs may share a similar substrate recognition mechanism with Wzb. The current studies provide the structural basis for rational drug design against the pathogenic bacteria.
- 论文类型：
期刊论文
- 论文编号：
10
- 卷号：
281
- 期号：
28
- 页面范围：
19570-19577
- 是否译文：
否
- 发表时间：
2006-07-14
- 收录刊物：
SCI
- 发布期刊链接：
- 第一作者：
Lescop Ewen
- 通讯作者：
Jin Changwen
- 全部作者：
Hu Yunfei,Xu Huimin,Hu Wei,Chen Jun,Xia Bin
